{"id":4192,"date":"2016-02-17T19:54:36","date_gmt":"2016-02-17T19:54:36","guid":{"rendered":"http:\/\/sites.rochester.edu\/larracuente\/?page_id=4192"},"modified":"2016-05-26T20:07:38","modified_gmt":"2016-05-26T20:07:38","slug":"lab-protocols","status":"publish","type":"page","link":"https:\/\/sites.rochester.edu\/larracuente\/lab-protocols\/","title":{"rendered":"Lab protocols"},"content":{"rendered":"<p>Below are links to commonly used Larracuente Lab protocols.<\/p>\n<figure id=\"attachment_4322\" aria-describedby=\"caption-attachment-4322\" style=\"width: 173px\" class=\"wp-caption alignright\"><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec.jpeg\" rel=\"attachment wp-att-4322\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4322\" src=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec-300x208.jpeg\" alt=\"2_slot32sec\" width=\"173\" height=\"120\" srcset=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec-300x208.jpeg 300w, https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/2_slot32sec.jpeg 360w\" sizes=\"auto, (max-width: 173px) 100vw, 173px\" \/><\/a><figcaption id=\"caption-attachment-4322\" class=\"wp-caption-text\">Slot blot showing copy number variation of\u00a0 repeats in Drosophila melanogaster. Credit: D. Eickbush<\/figcaption><\/figure>\n<p><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Squish.Preps_.pdf\" rel=\"\">Squish prep <\/a><\/p>\n<p><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Squish_Plus_Preps.pdf\" rel=\"\">&#8220;Squish +&#8221; prep<\/a><\/p>\n<p><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Slot_blotting_DNA.pdf\" rel=\"\">DNA slot blots<\/a><\/p>\n<p><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.RNA_Isolation.pdf\" rel=\"\">Total RNA isolation<\/a><\/p>\n<p><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Chemiluminscence.pdf\" rel=\"\">Chemiluminescent hybridization and <\/a><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/LarracuenteLab.Chemiluminscence.pdf\" rel=\"\">detection<\/a><\/p>\n<figure id=\"attachment_796\" aria-describedby=\"caption-attachment-796\" style=\"width: 146px\" class=\"wp-caption alignright\"><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg.jpg\" rel=\"attachment wp-att-796\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-796\" src=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg.jpg\" alt=\"359_jpeg\" width=\"146\" height=\"146\" srcset=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg.jpg 168w, https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2014\/12\/359_jpeg-150x150.jpg 150w\" sizes=\"auto, (max-width: 146px) 100vw, 146px\" \/><\/a><figcaption id=\"caption-attachment-796\" class=\"wp-caption-text\">FISH showing the hybridization of a 1.688 satellite DNA probe on D. melanogaster mitotic chromosomes.<\/figcaption><\/figure>\n<p><a href=\"https:\/\/sites.rochester.edu\/larracuente\/wp-content\/uploads\/2016\/02\/Larracuente_Ferree_2015.pdf\" rel=\"\">FISH on squashed tissue<\/a><\/p>\n<p>&nbsp;<\/p>\n<h3><strong>Computational protocols<\/strong><\/h3>\n<p>Please visit our GitHub page for computational pipelines: <a href=\"https:\/\/github.com\/LarracuenteLab\/\" target=\"_blank\">https:\/\/github.com\/LarracuenteLab\/<\/a>.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Below are links to commonly used Larracuente Lab protocols. Squish prep &#8220;Squish +&#8221; prep DNA slot blots Total RNA isolation Chemiluminescent hybridization and detection FISH on squashed tissue &nbsp; Computational&hellip;<\/p>\n","protected":false},"author":26,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_members_access_role":[],"_members_access_error":""},"class_list":["post-4192","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Lab protocols - Larracuente Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.rochester.edu\/larracuente\/lab-protocols\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Lab protocols - Larracuente Lab\" \/>\n<meta property=\"og:description\" content=\"Below are links to commonly used Larracuente Lab protocols. 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